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ar tic le in pr es s alpl  (Proteintech)


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    Structured Review

    Proteintech ar tic le in pr es s alpl
    Ar Tic Le In Pr Es S Alpl, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+alpl/ALPL+Antibody/pm41872929-122-2-14
    Average 96 stars, based on 201 article reviews
    ar tic le in pr es s alpl - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Caldesmon-1–mediated actin dynamics is essential for osteogenic differentiation of aortic valve interstitial cells
    Article Snippet: Membranes were incubated with primary antibodies, including anti-CALD1 (Proteintech, Catalogue no. 66693-I, 1:4000 dilution), anti-phosphoMLC (Cell Signaling Technology, Catalogue no. 3671, 1:5000 dilution), and anti-ACTB (Sigma-Aldrich, Catalogue no. A5441, 1:4000 dilution). .. Lysates (20 μg) from VICs cultured with or without osteogenic medium for 14 days were collected and analysed by western blotting using primary antibodies, including anti-ALPL (Proteintech, Cat. No. 11187-1-AP, 1:500 dilution), anti-RUNX2 (Cell Signaling Technology, Catalogue no. 12556 S, 1:500 dilution), and anti-α-Tubulin (FUJIFILM Wako, Catalogue no. 013-25033, 1:1000 dilution). .. After primary antibody incubation, membranes were treated with the appropriate secondary antibodies: anti-Mouse IgG (H + L), HRP Conjugate (Promega, Catalogue no. W4021) for CALD1, α-Tubulin and ACTB, and anti-Rabbit IgG (H + L), HRP Conjugate (Promega, Catalogue no. W4011) for pMLC, RUNX2, and ALPL.

    Article Title: Caldesmon-1-mediated actin dynamics is essential for osteogenic differentiation of aortic valve interstitial cells.
    Article Snippet: Membranes were incubated with primary antibodies, including anti-CALD1 (Proteintech, Catalogue no. 66693-I, 1:4000 dilution), anti-phosphoMLC (Cell Signaling Technology, Catalogue no. 3671, 1:5000 dilution), and anti-ACTB (Sigma-Aldrich, Catalogue no. A5441, 1:4000 dilution). .. Lysates (20 μg) from VICs cultured with or without osteogenic medium for 14 days were collected and analysed by western blotting using primary antibodies, including anti-ALPL (Proteintech, Cat. No. 11187-1-AP, 1:500 dilution), anti-RUNX2 (Cell Signaling Technology, Catalogue no. 12556S, 1:500 dilution), and anti-α-Tubulin (FUJIFILM Wako, Catalogue no. 013-25033, 1:1000 dilution). .. After primary antibody incubation, membranes were treated with the appropriate secondary antibodies: anti-Mouse IgG (H+L), HRP Conjugate (Promega, Catalogue no. W4021) for CALD1, α-Tubulin and ACTB, and anti-Rabbit IgG (H+L), HRP Conjugate (Promega, Catalogue no. W4011) for pMLC, RUNX2, and ALPL.

    Western Blot:

    Article Title: Caldesmon-1–mediated actin dynamics is essential for osteogenic differentiation of aortic valve interstitial cells
    Article Snippet: Membranes were incubated with primary antibodies, including anti-CALD1 (Proteintech, Catalogue no. 66693-I, 1:4000 dilution), anti-phosphoMLC (Cell Signaling Technology, Catalogue no. 3671, 1:5000 dilution), and anti-ACTB (Sigma-Aldrich, Catalogue no. A5441, 1:4000 dilution). .. Lysates (20 μg) from VICs cultured with or without osteogenic medium for 14 days were collected and analysed by western blotting using primary antibodies, including anti-ALPL (Proteintech, Cat. No. 11187-1-AP, 1:500 dilution), anti-RUNX2 (Cell Signaling Technology, Catalogue no. 12556 S, 1:500 dilution), and anti-α-Tubulin (FUJIFILM Wako, Catalogue no. 013-25033, 1:1000 dilution). .. After primary antibody incubation, membranes were treated with the appropriate secondary antibodies: anti-Mouse IgG (H + L), HRP Conjugate (Promega, Catalogue no. W4021) for CALD1, α-Tubulin and ACTB, and anti-Rabbit IgG (H + L), HRP Conjugate (Promega, Catalogue no. W4011) for pMLC, RUNX2, and ALPL.

    Article Title: Caldesmon-1-mediated actin dynamics is essential for osteogenic differentiation of aortic valve interstitial cells.
    Article Snippet: Membranes were incubated with primary antibodies, including anti-CALD1 (Proteintech, Catalogue no. 66693-I, 1:4000 dilution), anti-phosphoMLC (Cell Signaling Technology, Catalogue no. 3671, 1:5000 dilution), and anti-ACTB (Sigma-Aldrich, Catalogue no. A5441, 1:4000 dilution). .. Lysates (20 μg) from VICs cultured with or without osteogenic medium for 14 days were collected and analysed by western blotting using primary antibodies, including anti-ALPL (Proteintech, Cat. No. 11187-1-AP, 1:500 dilution), anti-RUNX2 (Cell Signaling Technology, Catalogue no. 12556S, 1:500 dilution), and anti-α-Tubulin (FUJIFILM Wako, Catalogue no. 013-25033, 1:1000 dilution). .. After primary antibody incubation, membranes were treated with the appropriate secondary antibodies: anti-Mouse IgG (H+L), HRP Conjugate (Promega, Catalogue no. W4021) for CALD1, α-Tubulin and ACTB, and anti-Rabbit IgG (H+L), HRP Conjugate (Promega, Catalogue no. W4011) for pMLC, RUNX2, and ALPL.

    Article Title: In Vivo and In Vitro Analysis of Cathepsin D in Bone Homeostasis and Osteoporosis
    Article Snippet: Lysates were centrifuged at 15,000 × g for 10 minutes at 4°C, and protein concentrations were determined using a BCA protein assay kit (P0010, Beyotime). .. Membranes were blocked with Western blocking solution (P0023B, Beyotime) for 90 minutes at room temperature and then incubated overnight at 4°C with the following primary antibodies: anti-ALPL (11187-1-AP, Proteintech), anti-RUNX2 ( SEM29595 , SAB), anti-CTSD (ab75811, Abcam), anti-COL1A1 (AF7001, Affinity), and anti-GAPDH (AF7021, Affinity). .. Membranes were blocked with Western blocking solution (P0023B, Beyotime) for 90 minutes at room temperature and then incubated overnight at 4°C with the following primary antibodies: anti-ALPL (11187-1-AP, Proteintech), anti-RUNX2 ( SEM29595 , SAB), anti-CTSD (ab75811, Abcam), anti-COL1A1 (AF7001, Affinity), and anti-GAPDH (AF7021, Affinity).

    Blocking Assay:

    Article Title: In Vivo and In Vitro Analysis of Cathepsin D in Bone Homeostasis and Osteoporosis
    Article Snippet: Lysates were centrifuged at 15,000 × g for 10 minutes at 4°C, and protein concentrations were determined using a BCA protein assay kit (P0010, Beyotime). .. Membranes were blocked with Western blocking solution (P0023B, Beyotime) for 90 minutes at room temperature and then incubated overnight at 4°C with the following primary antibodies: anti-ALPL (11187-1-AP, Proteintech), anti-RUNX2 ( SEM29595 , SAB), anti-CTSD (ab75811, Abcam), anti-COL1A1 (AF7001, Affinity), and anti-GAPDH (AF7021, Affinity). .. Membranes were blocked with Western blocking solution (P0023B, Beyotime) for 90 minutes at room temperature and then incubated overnight at 4°C with the following primary antibodies: anti-ALPL (11187-1-AP, Proteintech), anti-RUNX2 ( SEM29595 , SAB), anti-CTSD (ab75811, Abcam), anti-COL1A1 (AF7001, Affinity), and anti-GAPDH (AF7021, Affinity).

    Incubation:

    Article Title: In Vivo and In Vitro Analysis of Cathepsin D in Bone Homeostasis and Osteoporosis
    Article Snippet: Lysates were centrifuged at 15,000 × g for 10 minutes at 4°C, and protein concentrations were determined using a BCA protein assay kit (P0010, Beyotime). .. Membranes were blocked with Western blocking solution (P0023B, Beyotime) for 90 minutes at room temperature and then incubated overnight at 4°C with the following primary antibodies: anti-ALPL (11187-1-AP, Proteintech), anti-RUNX2 ( SEM29595 , SAB), anti-CTSD (ab75811, Abcam), anti-COL1A1 (AF7001, Affinity), and anti-GAPDH (AF7021, Affinity). .. Membranes were blocked with Western blocking solution (P0023B, Beyotime) for 90 minutes at room temperature and then incubated overnight at 4°C with the following primary antibodies: anti-ALPL (11187-1-AP, Proteintech), anti-RUNX2 ( SEM29595 , SAB), anti-CTSD (ab75811, Abcam), anti-COL1A1 (AF7001, Affinity), and anti-GAPDH (AF7021, Affinity).



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    Image Search Results


    Primary antibody and detection kits used in immunochemistry.

    Journal: Cells

    Article Title: Inhibiting TNAP Attenuates the Aortic Valve Calcification and Reduces Oxidative Stress

    doi: 10.3390/cells15171543

    Figure Lengend Snippet: Primary antibody and detection kits used in immunochemistry.

    Article Snippet: Anti-ALPL antibody , ABclonal, Woburn, MA, USA , 1:100.

    Techniques: Plasmid Preparation